Degradative potential of Stenotrophomonas strain HPC383 having genes homologous to dmp operon

Bioresour Technol. 2011 Feb;102(3):3227-33. doi: 10.1016/j.biortech.2010.11.016. Epub 2010 Nov 12.

Abstract

A strain, Stenotrophomonas HPC383 is isolated from effluent treatment plant treating wastewater from pesticide industry; degrades various aromatic compounds (cresols, phenol, catechol, 4methyl-catechol and hydroquinone) and crude oil, as determined through HPLC and GC analysis. Culture HPC383 could degrade (%) various compounds (1 mM) from a mixture: phenol - 99, p-cresol - 100, 4-methylcatechol - 96 and hydroquinone - 43 within 48 h of incubation, whereas it took 7 days to degrade 94% of 0.5% crude oil. Gene locus dmpN, to identify phenol degrading capacity was determined by PCR followed by southern analysis. The sequenced DNA fragment exhibited 99% sequence similarity to phenol hydroxylase gene from Arthrobacter sp. W1 (FJ610336). Amino acid sequence analysis of phenol hydroxylase reveals it to belong to high-Ks (affinity constant) group. Application of HPC383 in bioremediation of aquatic and terrestrial sites contaminated with petrochemical has been suggested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biodegradation, Environmental
  • Hydrocarbons, Aromatic / metabolism*
  • Mixed Function Oxygenases / genetics*
  • Molecular Sequence Data
  • Operon / genetics
  • Pesticides / metabolism*
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Stenotrophomonas / chemistry
  • Stenotrophomonas / classification*
  • Stenotrophomonas / physiology*
  • Water Pollutants, Chemical / metabolism*
  • Water Purification / methods*

Substances

  • Hydrocarbons, Aromatic
  • Pesticides
  • Water Pollutants, Chemical
  • Mixed Function Oxygenases
  • phenol 2-monooxygenase