Abstract
The methanolic extract of the roots of Rubia akane (Rubiaceae) was found to show inhibitory activity on phosphatase of regenerating liver-3 (PRL-3). Bioassay-guided fractionation of the methanolic extract resulted in the isolation of two anthraquinone compounds, 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-α-rhamnosyl(1→2)-β-glucoside and 2-methyl-1,3,6-trihydroxy-9,10-anthraquinone, as inhibitors on PRL-3. These compounds inhibited PRL-3 in a dose-dependent manner with IC₅₀ values of 5.2 and 1.3 μg/mL, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anthraquinones / analysis
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Anthraquinones / isolation & purification*
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Anthraquinones / metabolism
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Anthraquinones / pharmacology*
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology*
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Glucosides / analysis
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Glucosides / isolation & purification*
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Glucosides / metabolism
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Glucosides / pharmacology*
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Humans
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Neoplasm Proteins / antagonists & inhibitors*
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Neoplasm Proteins / metabolism
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Plant Roots / chemistry
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Protein Tyrosine Phosphatases / antagonists & inhibitors*
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Protein Tyrosine Phosphatases / metabolism
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Rubia / chemistry*
Substances
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2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-(6'-O-acetyl)-alpha-rhamnosyl(1-2)-beta-glucoside
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Anthraquinones
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Antineoplastic Agents
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Glucosides
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Neoplasm Proteins
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PTP4A3 protein, human
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Protein Tyrosine Phosphatases