Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8'-hydroxylase CYP707A

Mariko Okazaki; Hataitip Nimitkeatkai; Taku Muramatsu; Hikaru Aoyama; Kotomi Ueno; Masaharu Mizutani; Nobuhiro Hirai; Satoru Kondo; Toshiyuki Ohnishi; Yasushi Todoroki

doi:10.1016/j.bmc.2010.11.011

Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8'-hydroxylase CYP707A

Bioorg Med Chem. 2011 Jan 1;19(1):406-13. doi: 10.1016/j.bmc.2010.11.011. Epub 2010 Nov 10.

Authors

Mariko Okazaki¹, Hataitip Nimitkeatkai, Taku Muramatsu, Hikaru Aoyama, Kotomi Ueno, Masaharu Mizutani, Nobuhiro Hirai, Satoru Kondo, Toshiyuki Ohnishi, Yasushi Todoroki

Affiliation

¹ Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.

PMID: 21115253
DOI: 10.1016/j.bmc.2010.11.011

Abstract

We developed abscinazole-E1 (Abz-E1), a specific inhibitor of abscisic acid (ABA) 8'-hydroxylase (CYP707A). This inhibitor was designed and synthesized as an enlarged analogue of uniconazole (UNI), a well-known plant growth retardant, which inhibits a gibberellin biosynthetic enzyme (ent-kaurene oxidase, CYP701A) as well as CYP707A. Our results showed that Abz-E1 functions as a potent inhibitor of CYP707A and a poor inhibitor of CYP701A both in vitro and in vivo. Abz-E1 application to plants resulted in improved desiccation tolerance and an increase in endogenous ABA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / enzymology
Arabidopsis / physiology
Cytochrome P-450 Enzyme System / chemistry*
Cytochrome P-450 Enzyme System / metabolism*
Germination
Oryza / enzymology
Oryza / physiology
Plant Proteins
Triazoles / chemistry*

Substances

Plant Proteins
Triazoles
abscinazole E1
Cytochrome P-450 Enzyme System
abscisic acid 8'-hydroxylase