Abstract
Cholecystokinin (CCK) activates the myenteric neurons of adult rats. The goal of this work is to determine the ontogeny of this activation by CCK-8 in the myenteric plexus of the duodenum (2cm immediately following the pyloric sphincter aborally) and compare it with that of the dorsal vagal complex (DVC) - which occurs in 1-day old pups. Despite the existence of both of the CCK receptors, CCK(1) and CCK(2), in 4, 14, 21 and 35 day old rats, CCK-8 (0, 5, 10, 20 and 40μg/kg, i.p.) increased Fos-like immunoreactivity (Fos-LI, a marker for neuronal activation) in the myenteric neurons of 21- and 35-day old rats but in the DVC of all age groups. As such, this belated activation of myenteric neurons by CCK-8 compared to the DVC may reflect a delayed role for these neurons in CCK-related functions.
Published by Elsevier Inc.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Age Factors
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Animals
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Animals, Newborn
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Cell Count
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Cell Size
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Cholecystokinin / pharmacology*
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Duodenum / drug effects
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Duodenum / growth & development*
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Duodenum / innervation*
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Duodenum / metabolism
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Female
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Gene Expression / genetics
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Male
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Myenteric Plexus / cytology
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Myenteric Plexus / drug effects
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Myenteric Plexus / growth & development*
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Myenteric Plexus / metabolism
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Neurons / cytology
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Neurons / drug effects*
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Neurons / metabolism
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Oncogene Proteins v-fos / metabolism
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Peptide Fragments / pharmacology*
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Rats
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Rats, Sprague-Dawley
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Receptor, Cholecystokinin A / genetics
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Receptor, Cholecystokinin B / genetics
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Solitary Nucleus / drug effects
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Solitary Nucleus / metabolism
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Vagus Nerve / drug effects
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Vagus Nerve / metabolism
Substances
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Oncogene Proteins v-fos
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Peptide Fragments
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Receptor, Cholecystokinin A
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Receptor, Cholecystokinin B
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cholecystokinin 8
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Cholecystokinin