The advancement of both LC and MS has contributed significantly to phospholipid analysis. Two major trends of developments have emerged in the past decade: application of dedicated online (or offline) LC-MS techniques including 2D and sophisticated chromatographic separations, and the development of so-called shotgun lipidomics represented by multidimensional MS-based techniques. However, neither of these techniques have been shown to be a universal solution for the increasing demand on the comprehensive information of lipid metabolomics in lipidomics studies. This is partially due to the intrinsic complexity of naturally occurring phospholipids in practice. It is evident that either chromatography or MS has to go multidimensional in order to fulfil this goal. This review focuses on recent developments of multidimensional MS, LC-MS and chromatographic approaches for lipidomics analysis. The perspectives and retrospectives of chromatography and MS in these aspects will be reviewed and discussed.