Rapid DNA detection by interface PCR on nanoparticles

Hua Kuang; Shuge Zhao; Wei Chen; Wei Ma; Qianqian Yong; Liguang Xu; Libing Wang; Chuanlai Xu

doi:10.1016/j.bios.2010.10.043

Rapid DNA detection by interface PCR on nanoparticles

Biosens Bioelectron. 2011 Jan 15;26(5):2495-9. doi: 10.1016/j.bios.2010.10.043. Epub 2010 Oct 31.

Authors

Hua Kuang¹, Shuge Zhao, Wei Chen, Wei Ma, Qianqian Yong, Liguang Xu, Libing Wang, Chuanlai Xu

Affiliation

¹ School of Food Science and Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214122, PR China.

PMID: 21081272
DOI: 10.1016/j.bios.2010.10.043

Abstract

A novel, rapid DNA detection method based on fluorescence quenching of quantum dots (QDs) by gold nanoparticles (GNPs) through polymerase chain reaction (PCR) was developed. In proof-of-concept experiments, the length of the amplicon DNA ranging from 152 to 1003 base pairs (bp) could be determined based on quenched fluorescence intensity with 136 bp as the lower limit of effective range. And the real sample detections were also achieved successfully by this developed method. Therefore, this DNA detection method has the potential to be the powerful gene diagnostic tool.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / instrumentation*
DNA / analysis
DNA / genetics*
Equipment Design
Equipment Failure Analysis
Gold / chemistry
Nanoparticles / chemistry*
Polymerase Chain Reaction / instrumentation*
Quantum Dots*
Sequence Analysis, DNA / instrumentation*
Spectrometry, Fluorescence / instrumentation*

Substances

Gold
DNA