Solid core column technology applied to HPLC-FD of paralytic shellfish toxins

Stacey L DeGrasse; Jeffrey A DeGrasse; Kevin Reuter

doi:10.1016/j.toxicon.2010.11.004

Solid core column technology applied to HPLC-FD of paralytic shellfish toxins

Toxicon. 2011 Jan;57(1):179-82. doi: 10.1016/j.toxicon.2010.11.004. Epub 2010 Nov 13.

Authors

Stacey L DeGrasse¹, Jeffrey A DeGrasse, Kevin Reuter

Affiliation

¹ US FDA, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, Division of Analytical Chemistry, Spectroscopy and Mass Spectrometry Branch, 5100 Paint Branch Parkway, HFS-707, College Park, MD 20740, USA. Stacey.DeGrasse@fda.hhs.gov

PMID: 21078334
DOI: 10.1016/j.toxicon.2010.11.004

Abstract

Pre-column oxidation liquid chromatography with fluorescence detection is a chemical method for analyzing paralytic shellfish toxins. In order to improve the sample throughput and efficiency of AOAC Method 2005.06, solid core particle column technology was evaluated. We demonstrate that supplanting the original fully porous particle column with a solid core particle column reduces sample analysis time from 15 to 5 min per sample and improves resolution.

Published by Elsevier Ltd.

MeSH terms

Animals
Chromatography, High Pressure Liquid
Food Contamination / analysis*
Harmful Algal Bloom
High-Throughput Screening Assays
Marine Toxins / analysis*
Phytoplankton
Shellfish / analysis*
Shellfish Poisoning
Solid Phase Extraction / methods

Substances

Marine Toxins