Landscape features of a watershed are important factors affecting non-point source (NPS) pollution. Sub-watershed bounds were delineated and landscape heterogeneity was analyzed based on GIS and RS in Xitiaoxi watershed which located the upper reach of Taihu Lake area. Nutrient export intensity of sub-watersheds was estimated by revised export coefficient model. Then the relationships between nutrient export and main landscape types, as well as Shannon diversity index (SHDI) in sub-watershed units were analyzed. Results show, TN and TP export intensity have obvious spatial difference, which changed from 3.01 kg/(hm2 x a) to 15.44 kg/(hm2 x a) and 0.049 kg/(hm2 x a) to 0.355 kg/(hm2 x a) respectively. The dominated landscape types including cultivated land and forest land quantitatively related with nutrient export intensity. TN and TP export intensity will decrease 0.203 1 kg/(hm2 x a) and 0.0152 kg/(hm2 x a) respectively with 10% increased of forest area, and will increase 0.5726 kg/(hm2 x a) and 0.0273 kg/(hm2 x a) with 10% increased of cultivated land area. The relationship between nutrient export intensity and SHDI exhibited second-degree polynomial, export intensity increased by SHDI increasing and to maximum when SHDI equals 1.5, then decreased with SHDI increasing. This research results will provide an important reference value for NPS management.