[Statistical analysis of distribution of antibody level against Mycobacterium bovis antigens for bovine tuberculosis diagnostics]

T A Redchuk; N V Korotkevich; A A Kaberniuk; E S Oleĭnik; A Iu Labyntsev; S I Romaniuk; D V Kolibo; V A Busol; S V Komisarenko

[Statistical analysis of distribution of antibody level against Mycobacterium bovis antigens for bovine tuberculosis diagnostics]

Tsitol Genet. 2010 Sep-Oct;44(5):23-30.

[Article in Russian]

Authors

T A Redchuk, N V Korotkevich, A A Kaberniuk, E S Oleĭnik, A Iu Labyntsev, S I Romaniuk, D V Kolibo, V A Busol, S V Komisarenko

PMID: 21061689

Abstract

Antibody responses to purified protein derivate PPD of tuberculin and to antigens MPB63 and MPB83 of Mycobacterium bovis were determined in bovine herd (94 adult animals). Statistical approach based on approximation by multiple Gaussians with Levenberg-Marquardt algorithm for analysis of antibody level distribution against antigens examined was provided. Our results confirm that indirect ELISA with recombinant MPB83 and MPB63 as well as conventional PPD could be used for test-systems development for detection of cow tuberculosis infection at the herd level.

Publication types

English Abstract

MeSH terms

Animals
Antibodies, Bacterial / blood*
Antigens, Bacterial / immunology*
Cattle
Cattle Diseases / diagnosis*
Cattle Diseases / microbiology
Immunoenzyme Techniques / methods
Immunoenzyme Techniques / statistics & numerical data*
Mycobacterium bovis / immunology*
Recombinant Proteins / immunology
Statistical Distributions*
Tuberculosis, Bovine / diagnosis*
Tuberculosis, Bovine / microbiology

Substances

Antibodies, Bacterial
Antigens, Bacterial
Recombinant Proteins