Abstract
This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Conjugation, Genetic
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DNA, Bacterial / isolation & purification
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Electroporation
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Escherichia coli / genetics
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Gene Library
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Genetic Engineering / methods*
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Genetics, Microbial / methods*
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Polymerase Chain Reaction / methods
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Protoplasts
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Streptomyces / cytology
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Streptomyces / genetics*
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Transformation, Genetic