Pseudomonas bauzanensis sp. nov., isolated from soil

Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2333-2337. doi: 10.1099/ijs.0.026104-0. Epub 2010 Oct 29.

Abstract

A Gram-negative, aerobic, motile rod, designated BZ93(T), was isolated from soil from an industrial site. The strain grew at 5-30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ93(T) was related to members of the genus Pseudomonas and was related most closely to Pseudomonas xiamenensis C10-2(T) (97.8 % 16S rRNA gene sequence similarity) and Pseudomonas pertucinogena IFO 14163(T) (97.4 %). The predominant cellular fatty acids of strain BZ93(T) were C(18 : 1)ω7c (54.8 %), summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH; 10.3 %), C(16 : 0) (9.9 %) and C(17 : 0) cyclo (7.4 %). The major quinone was ubiquinone 9. The major phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. The genomic DNA G+C content was 61.8 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Pseudomonas bauzanensis sp. nov., is proposed. The type strain is BZ93(T) ( = DSM 22558(T) = CGMCC 1.9095(T) = LMG 26048(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Bacterial Typing Techniques
  • Base Composition
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Locomotion
  • Molecular Sequence Data
  • Phospholipids / analysis
  • Phylogeny
  • Pseudomonas / classification*
  • Pseudomonas / genetics
  • Pseudomonas / isolation & purification*
  • Pseudomonas / physiology
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Phospholipids
  • Quinones
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/GQ161991