Abstract
A novel RNA component with oxidoreductase activity (diaphorase activity) has been purified from an RNA fraction of Torula yeast. The RNA component was obtained in a 0.05% yield by a series of steps, SDS-phenol extraction, nuclease P1 digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS-column.
MeSH terms
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Alkaline Phosphatase / metabolism
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Chromatography, High Pressure Liquid
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Chromatography, Ion Exchange
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Cryptococcus / enzymology*
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Dihydrolipoamide Dehydrogenase / metabolism*
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RNA, Catalytic / isolation & purification
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RNA, Catalytic / metabolism*
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RNA, Fungal / isolation & purification
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RNA, Fungal / metabolism
Substances
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RNA, Catalytic
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RNA, Fungal
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Dihydrolipoamide Dehydrogenase
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Alkaline Phosphatase