Abstract
Mechanical loading of muscles by intrinsic muscle activity or passive stretch leads to an increase in the production of reactive oxygen species. The NAD-dependent protein deacetylase SIRT1 is involved in the protection against oxidative stress by enhancing FOXO-driven Sod2 transcription. In this report, we unravel a mechanism triggered by mechanical stretch of skeletal muscle cells that leads to an EGR1-dependent transcriptional activation of the Sirt1 gene. The resulting transient increase in SIRT1 expression generates an antioxidative response that contributes to reactive oxygen species scavenging.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antioxidants / metabolism*
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Cell Line
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Early Growth Response Protein 1 / genetics
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Early Growth Response Protein 1 / metabolism*
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Humans
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Mice
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Muscle Cells / metabolism
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Muscle Proteins / genetics
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Muscle Proteins / metabolism*
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Muscle Stretching Exercises
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Muscle, Skeletal / metabolism*
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Oxidative Stress / physiology
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Reactive Oxygen Species / metabolism
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Sirtuin 1 / biosynthesis*
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Sirtuin 1 / genetics
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Superoxide Dismutase / biosynthesis*
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Superoxide Dismutase / genetics
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Transcription, Genetic / physiology
Substances
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Antioxidants
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Early Growth Response Protein 1
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Egr1 protein, mouse
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Muscle Proteins
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Reactive Oxygen Species
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Superoxide Dismutase
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superoxide dismutase 2
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Sirt1 protein, mouse
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Sirtuin 1