Kroppenstedtia eburnea gen. nov., sp. nov., a thermoactinomycete isolated by environmental screening, and emended description of the family Thermoactinomycetaceae Matsuo et al. 2006 emend. Yassin et al. 2009

Int J Syst Evol Microbiol. 2011 Sep;61(Pt 9):2304-2310. doi: 10.1099/ijs.0.026179-0. Epub 2010 Oct 22.

Abstract

A Gram-positive, spore-forming, aerobic, filamentous bacterium, strain JFMB-ATE(T), was isolated in 2008 during environmental screening of a plastic surface in grade C in a contract manufacturing organization in southern Germany. The isolate grew at temperatures of 25-50 °C and at pH 5.0-8.5, forming ivory-coloured colonies with sparse white aerial mycelia. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the family Thermoactinomycetaceae, except that the cell-wall peptidoglycan contained LL-diaminopimelic acid, while all previously described members of this family display this diagnostic diamino acid in meso-conformation. The DNA G+C content of the novel strain was 54.6 mol%, the main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, and the major menaquinone was MK-7. The major fatty acids had saturated C₁₄-C₁₆ branched chains. No diagnostic sugars were detected. Based on the chemotaxonomic results and 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel genus and species, Kroppenstedtia eburnea gen. nov. sp. nov. The type strain is JFMB-ATE(T) ( = DSM 45196(T) = NRRL B-24804(T) = CCUG 59226(T)).

MeSH terms

  • Bacillales / classification*
  • Bacillales / genetics
  • Bacillales / isolation & purification*
  • Bacterial Typing Techniques
  • Base Composition
  • Cell Wall / chemistry
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diaminopimelic Acid / analysis
  • Environmental Microbiology*
  • Germany
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Peptidoglycan / chemistry
  • Phospholipids / analysis
  • Phylogeny
  • Pigments, Biological / metabolism
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Spores, Bacterial / cytology
  • Temperature

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Peptidoglycan
  • Phospholipids
  • Pigments, Biological
  • Quinones
  • RNA, Ribosomal, 16S
  • Diaminopimelic Acid

Associated data

  • GENBANK/FN665656