Resolution improvement in optical projection tomography by the focal scanning method

Opt Lett. 2010 Oct 15;35(20):3363-5. doi: 10.1364/OL.35.003363.

Abstract

Optical projection tomography (OPT) requires the depth of field (DOF) of the lens to cover at least half of the sample. There is a trade-off between obtaining high resolution with a high-NA lens and obtaining large DOF with a low-NA lens. The DOF of a high-NA objective lens can be extended by scanning its focal plane through the sample. We call this extended DOF image a "pseudoprojection." Images reconstructed from these pseudoprojections have isometric resolution, which can be the same as the lateral resolution of the high-NA objective. The focal scanning method produces an over 10× improvement in OPT resolution.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Animals
  • Cell Line
  • Cytological Techniques / methods*
  • Image Processing, Computer-Assisted / methods
  • Imaging, Three-Dimensional / methods
  • Microscopy, Fluorescence / methods*
  • Muntjacs
  • Staining and Labeling
  • Tomography, Optical / methods*