Background and objective: BAG genes (Bcl-2-associated athanogene) belong to a recently discovered multifunctional anti-apoptosis gene family that regulate various physiological processes which include apoptosis, tumorigenesis, neural differentiation, stress response and cell cycle and so on. The expression status of BAG family genes are related to certain tumor incidence and prognosis. The aim of this study is to explore the association of the BAG family gene expression status with the susceptibility of lung adenocarcinoma.
Methods: The gene expression data of BAG family genes from 29 cases of lung adenocarcinoma tissues and matched pericancerous lung tissues were generated by microarray chips. Cox regression was used to analyze the association between the expression of BAG family genes and the susceptibility of lung adenocarcinoma and the results were verified by GEO database.
Results: The expression levels of BAG-1, BAG-2, BAG-5 in cancer tissues were significantly downregulated compared with matched pericancerous lung tissues and were protective factors of lung adenocarcinoma (P < 0.05, OR < 1); while the expression level of BAG-4 in cancer tissues were remarkably upregulated compared with the matched pericancerous lung tissues and was risk factor of lung adenocarcinoma (P < 0.05, OR > 1).
Conclusions: BAG-1, BAG-2, BAG-5 might be the potential protective factors while BAG-4 is possible risk factor of lung adenocarcinoma.
背景与目的: BAG（Bcl-2-associated athanogene）家族是一个多功能的抗凋亡家族，其表达与多种肿瘤的发病及预后相关。BAG基因是近年发现的抗凋亡基因家族，调节各种生理过程，包括细胞凋亡、肿瘤发生、神经分化、应激反应和细胞周期等。BAG家族的表达状态与某些肿瘤的发病和预后相关。本研究的目的是探讨BAG家族基因表达水平与肺腺癌易感性的关系，在分子水平探讨肺腺癌发病的相关危险因素。
方法: 采用基因表达谱芯片技术检测29例肺腺癌患者癌组织及癌旁肺组织BAG家族基因的表达，应用配对t检验和Cox回归拟合单因素条件Logistic回归方法。并利用美国GEO数据库验证相关结果。
结果: BAG-1、BAG-2、BAG-5基因癌旁肺组织中表达高于癌组织且为肺腺癌发生的保护因素（P < 0.05, OR < 1）；BAG-4基因在癌组织中表达高于癌旁肺组织且为肺腺癌发生的危险因素（P < 0.05, OR>1）；BAG-3基因在癌组织及癌旁正常组织表达差别无统计学意义（P>0.05）。
结论: BAG-1、BAG-2、BAG-5基因可能是为肺腺癌发生的保护性因素，BAG-4基因可能是为肺腺癌发生的危险性因素。