Abstract
CyaY is the bacterial homolog of frataxin, proposed to be involved in the assembly of iron-sulfur clusters. While, the physiological iron donor for the iron-sulfur clusters assembly remains controversial. In this study, the gene of CyaY from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the protein was purified by one-step affinity chromatography to homogeneity. The CyaY protein can bind ferric iron and serve as an iron donor for the biogenesis of iron-sulfur clusters on the scaffold protein IscU in the presence of IscS and L-cysteine in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acidithiobacillus / enzymology*
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Acidithiobacillus / genetics
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Bacterial Proteins / metabolism*
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Chromatography, Affinity
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Cloning, Molecular
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Escherichia coli / genetics
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Ferric Compounds / metabolism
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Iron / metabolism
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Models, Molecular
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Molecular Chaperones / genetics
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Molecular Chaperones / isolation & purification
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Molecular Chaperones / metabolism*
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Molecular Sequence Data
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Protein Binding
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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Ferric Compounds
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Molecular Chaperones
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Recombinant Proteins
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Iron