Abstract
We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriological Techniques / methods*
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DNA Primers / genetics*
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Humans
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Mycobacterium bovis / classification
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Mycobacterium bovis / genetics
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Mycobacterium bovis / isolation & purification*
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Mycobacterium tuberculosis / classification
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / isolation & purification*
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Polymerase Chain Reaction / methods*
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Tuberculosis / diagnosis*
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Tuberculosis / microbiology