Speckle tracking echocardiography (STE) is a novel tool to analyze left ventricular (LV) deformation in standard grey-scale images. The aim of our study was to characterize global and regional LV function by STE in a large cohort of healthy adult probands across a wide range of age. 144 healthy individuals (71 men, 73 women; mean age 42 ± 14 years, range: 17-80 years) who had undergone a standard echocardiographic examination were retrospectively investigated by STE. Longitudinal strain (LS) was measured in three apical views, and radial strain (RS) in a short-axis view at papillary muscle level. Averaged global LS was -20.6 ± 2.6%, and averaged RS 48.2 ± 13.8%. On a regional basis, LS showed an increase from base to apex (basal vs. mid vs. apical segments: -18.5 ± 2.7% vs. -19.9 ± 2.6% vs. -22.9 ± 4.5%; P < 0.001). RS increased with age (P < 0.05), while LS decreased in basal, and slightly increased in the apical segments. LS and RS decreased with body surface area and LV size (P < 0.05). In the longitudinal dimension about 38% of the segments showed some degree of systolic pre-stretching, with a higher likelihood in basal segments (P = 0.02 vs. mid and apical segments). In the radial dimension pre-stretching was even more frequent (60%). STE offers a simple and rapid method to measure regional myocardial deformation. Our results, based on a large cohort of normal probands covering a wide range of ages, may serve as reference for comparison with different disease entities.