We demonstrate a tunable laser frequency comb operating near 420 nm with mode spacing of 20-50 GHz, usable bandwidth of 15 nm and output power per line of ~20 nW. Using the TRES spectrograph at the Fred Lawrence Whipple Observatory, we characterize this system to an accuracy below 1m/s, suitable for calibrating high-resolution astrophysical spectrographs used, e.g., in exoplanet studies.