A fluorescence-based Coomassie Blue protocol for two-dimensional gel-based proteomics

Yongxin Yang; Jiaqi Wang; Dengpan Bu; Leying Zhang; Shanshan Li; Lingyun Zhou; Hongyang Wei

doi:10.1007/s10529-010-0404-8

A fluorescence-based Coomassie Blue protocol for two-dimensional gel-based proteomics

Biotechnol Lett. 2011 Jan;33(1):119-21. doi: 10.1007/s10529-010-0404-8. Epub 2010 Sep 30.

Authors

Yongxin Yang¹, Jiaqi Wang, Dengpan Bu, Leying Zhang, Shanshan Li, Lingyun Zhou, Hongyang Wei

Affiliation

¹ State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China.

PMID: 20882320
DOI: 10.1007/s10529-010-0404-8

Abstract

A sensitive and convenient "visible SYPRO" staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive than that of Blue Silver, simpler than that of SYPRO Ruby, and compatible with subsequent mass spectrometry analysis.

Publication types

Evaluation Study

MeSH terms

Electrophoresis, Gel, Two-Dimensional / methods*
Electrophoresis, Polyacrylamide Gel / methods
Fluorescence
Proteomics / methods*
Rosaniline Dyes / metabolism*
Sensitivity and Specificity
Staining and Labeling / methods*

Substances

Rosaniline Dyes
Coomassie blue