Objective: To establish two-dimensional electrophoresis (2-DE) pattern for proteome analysis on K562 cells before and after treatment with 6-gingerol, and mass-spectrometry was applied to identify and analyze the differentially expressed proteins.
Methods: K562 cells were treated with 6-gingerol. Cell proliferation was analyzed by CCK-8 assay. Total protein of K562 cells was extracted by 2D-DIGE and then imaged by SDS gel scanning. The differentially expressed proteins were identified using Imagine Master 2D Platinum 6.0 software and functionally classified by MALDI and MALDI-TOF MS.
Results: 6-gingerol could significantly inhibit K562 cells proliferation and the efficacy was concentration and dose-dependent. After being treated for 24, 48, 72 h, IC50 was 22.86, 15.75, 11.18 microg/mL, respectively. 42 differentially expressed proteins were identified, including 19 up-regulated expressed proteins and 10 down-regulated expressed proteins.
Conclusion: Proteomic technique can be used to screen multiple proteins associate with the anti-leukemia effect of 6-gingerol, involving some important proteins related to oxidative stress, cell cycle regulation, apoptosis signal transduction, biosynthesis and glycometabolism.