Abstract
The performance of the VITEK 2 System (bioMérieux) version 3.01 software was compared to that of the E-test (AB Biodisk, Solna, Sweden) for antibiotic susceptibility testing of 17 clinical isolates of vancomycin resistant enterococcus (VRE). Antibiotic Susceptibility Testing (AST) by VITEK 2 produced 10 minor (59%) errors, resulting in false phenotypes. Reporting of vancomycin resistance in enterococcal strains has enormous therapeutic and epidemiological consequences. Therefore, at laboratories using automated systems (e.g. VITEK 2) for routine microbiological susceptibility testing data must be confirmed by independent validated methods, e.g. E-test, or microdilution.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Bacterial Typing Techniques / instrumentation
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Bacterial Typing Techniques / methods*
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Carbon-Oxygen Ligases / genetics
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Carbon-Oxygen Ligases / metabolism*
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Enterococcus / classification
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Enterococcus / drug effects
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Enterococcus / enzymology
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Enterococcus / isolation & purification*
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Genotype
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Glycopeptides / pharmacology*
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Gram-Positive Bacterial Infections / microbiology
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Humans
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Microbial Sensitivity Tests / instrumentation
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Microbial Sensitivity Tests / methods*
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Peptide Synthases / genetics
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Peptide Synthases / metabolism*
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Software
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Vancomycin Resistance
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Glycopeptides
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VanA ligase, Bacteria
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Carbon-Oxygen Ligases
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Peptide Synthases
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VanC protein, Enterococcus