Aim: To screen Foxp3Delta2-interacting proteins by yeast two-hybria.
Methods: pGBKT7-Foxp3Delta2 bait plasmid was constructed. Its toxicity and transcriptional activation in yeast cell AH109 was tested by observation of phenotypes and color.
Results: The bait plasmid pGBKT7-Foxp3Delta2 was constructed successfully and there was no self-activation or toxicity in AH109. Forty positive clones were obtained. After sequence comparison and analysis, nine candidate proteins were finally selected for further confirmation.
Conclusion: Yeast two hybrid system could be used for screening Foxp3Delta2-interacting proteins. These results provide essential clues for further investigation of Foxp3Delta2 function.