Ligand dimerization programmed by hybridization to study multimeric ligand-receptor interactions

Chem Commun (Camb). 2010 Nov 7;46(41):7742-4. doi: 10.1039/c0cc02852e. Epub 2010 Sep 20.

Abstract

Oligomerization of receptors induced or stabilized by polyvalent ligands is a fundamental mechanism in cellular recognition and signal transduction. Herein we report a general approach to encode complex peptide macrocycles with peptide nucleic acid (PNA) tags and program their oligomerization through hybridization as exemplified with a ligand binding to oligomeric DR5, a receptor of TRAIL cytokine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Dimerization*
  • Ligands
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptide Nucleic Acids / chemistry*
  • Peptide Nucleic Acids / metabolism
  • Protein Binding
  • Protein Multimerization*
  • Protein Structure, Quaternary
  • Receptors, TNF-Related Apoptosis-Inducing Ligand / chemistry*
  • Receptors, TNF-Related Apoptosis-Inducing Ligand / metabolism*
  • Surface Plasmon Resonance

Substances

  • Ligands
  • Peptide Nucleic Acids
  • Receptors, TNF-Related Apoptosis-Inducing Ligand