Abstract
Methyl (R)-2-chloromandelate, a key intermediate in the synthesis of clopidogrel, was obtained by the reduction of methyl-2-chlorobenzoylformate using whole cells of Saccharomyces cerevisiae. A 100% conversion and 96.1% of enantiomeric excess (ee) value was obtained when 17 methyl-2-chlorobenzoylformate/l was reacted with 8 g S. cerevisiae/l and 83 g glucose/l at pH 7.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biotransformation
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Glucose / metabolism
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Hydrogen-Ion Concentration
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Mandelic Acids / chemistry
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Mandelic Acids / metabolism*
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Phenylacetates / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Stereoisomerism
Substances
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Mandelic Acids
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Phenylacetates
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methyl 2-chlorobenozylformate
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methyl 2-chloromandelate
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Glucose