Diversity and abundance of zoonotic pathogens and indicators in manures of feedlot cattle in Australia

Appl Environ Microbiol. 2010 Oct;76(20):6947-50. doi: 10.1128/AEM.01095-10. Epub 2010 Aug 27.

Abstract

The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Escherichia coli, 10² to 10⁷ genome copies g⁻¹ (dry weight) manure were recovered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Australia
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Biodiversity*
  • Cattle
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • DNA, Protozoan / genetics
  • DNA, Protozoan / isolation & purification*
  • Manure / microbiology*
  • Parasites / classification
  • Parasites / genetics
  • Parasites / isolation & purification*
  • Polymerase Chain Reaction

Substances

  • DNA, Bacterial
  • DNA, Protozoan
  • Manure