Abstract
NMDA receptors are typically excited by a combination of glutamate and glycine. Here we describe excitatory responses in CNS myelin that are gated by a glycine agonist alone and mediated by NR1/NR3 "NMDA" receptor subunits. Response properties include activation by d-serine, inhibition by the glycine-site antagonist CNQX, and insensitivity to the glutamate-site antagonist d-APV. d-Serine responses were abrogated in NR3A-deficient mice. Our results suggest the presence of functional NR1/NR3 receptors in CNS myelin.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Central Nervous System / physiology
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Excitatory Postsynaptic Potentials / physiology*
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Glycine / physiology*
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Humans
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Mice
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Mice, Knockout
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Myelin Sheath / physiology*
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Protein Subunits / agonists
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Protein Subunits / genetics
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Protein Subunits / physiology*
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Rats
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Rats, Long-Evans
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Receptors, N-Methyl-D-Aspartate / agonists
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Receptors, N-Methyl-D-Aspartate / genetics
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Receptors, N-Methyl-D-Aspartate / physiology*
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Recombinant Proteins / agonists
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Recombinant Proteins / pharmacology
Substances
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NR1 NMDA receptor
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NR3A NMDA receptor
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Protein Subunits
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Receptors, N-Methyl-D-Aspartate
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Recombinant Proteins
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Glycine