A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method

J Fish Biol. 2009 Jul;75(1):268-75. doi: 10.1111/j.1095-8649.2009.02261.x.

Abstract

This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acidosis / blood
  • Acidosis / etiology
  • Acidosis / pathology
  • Acidosis / veterinary*
  • Animals
  • Carbon Dioxide* / analysis
  • Carbon Dioxide* / pharmacology
  • Erythrocytes / chemistry*
  • Erythrocytes / drug effects
  • Fish Diseases / blood
  • Fish Diseases / etiology
  • Fish Diseases / pathology*
  • Fishes / physiology
  • Freezing
  • Hydrogen-Ion Concentration
  • Intracellular Fluid / chemistry*
  • Reproducibility of Results
  • Specimen Handling / methods*
  • Specimen Handling / standards
  • Time Factors
  • Zoology / methods*

Substances

  • Carbon Dioxide