Objective: To evaluate the clinical significances of combined detections of the autoantibodies ANA, ANAs and anti-dsDNA in patients with systemic lupus erythematosus (SLE).
Methods: ANA was tested by indirect immunofluorescent method (IIF), ENA was tested by Western blot and anti-dsDNA was measured with radioimmunoassay in the serum samples from patients with SLE (n=90), and from the comparison group (n=74) and healthy control group (n=53).
Results: The sensitivity of anti-dsDNA, AnuA,anti-Sm in patients with SLE was 41.1%, 32.2% and 22.2%. The specificity of anti-dsDNA, AnuA, anti-Sm in SLE patients was all 97.3%. The positive likelihood ratios were 15.22, 11.93 and 8.22, respectively. The sensitivity and specificity reached 62.2% and 90.5%, respectively by combined determinations of anti-dsDNA, AnuA and anti-Sm.
Conclusion: The combined examinations of anti-dsDNA, AnuA, anti-Sms can improve the sensitivity and efficiency of laboratory diagnosis of SLE.