Water concentration and distribution in biotissues are important factors in many applications. THz-wave is a viable tool for water content measurement due to its highly sensitivity to water. In this study, the measuring errors of water concentration using THz-wave induced by transmittance and sample thickness were analyzed theoretically. The chosen basis for sample thickness and measuring THz frequency were presented theoretically. Measurements of the water two-dimensional mapping in different animal tissue samples were demonstrated experimentally, which clearly shows the spatial distribution of the tissues.