Monoclonal antibodies against the PB1-F2 protein of H1N1 influenza A virus

Hybridoma (Larchmt). 2010 Aug;29(4):321-6. doi: 10.1089/hyb.2010.0010.

Abstract

In this report we describe the generation of a mouse monoclonal antibody (MAb) against the influenza A virus PB1-F2 protein that is derived from a +1 reading frame of the polymerase basic protein (PB1) gene segment. We further present data that the hybridoma subclone F2-6G10 produces antibodies that specifically recognize the PB1-F2 protein of H1N1 influenza virus types only. The antibody can be used for immunodetection of the PB1-F2 protein in ELISA, Western blot, immunoprecipitation, and immunofluorescence assays.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Cells, Cultured
  • Dogs
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • HeLa Cells
  • Humans
  • Hybridomas
  • Immunoblotting
  • Immunoenzyme Techniques
  • Immunoglobulin G / immunology*
  • Immunoprecipitation
  • Kidney / cytology
  • Kidney / metabolism
  • Mice
  • Mice, Inbred BALB C
  • NIH 3T3 Cells
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology*
  • Recombinant Proteins / metabolism
  • Viral Proteins / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin G
  • PB1-F2 protein, Influenza A virus
  • Recombinant Proteins
  • Viral Proteins