Abstract
In this report we describe the generation of a mouse monoclonal antibody (MAb) against the influenza A virus PB1-F2 protein that is derived from a +1 reading frame of the polymerase basic protein (PB1) gene segment. We further present data that the hybridoma subclone F2-6G10 produces antibodies that specifically recognize the PB1-F2 protein of H1N1 influenza virus types only. The antibody can be used for immunodetection of the PB1-F2 protein in ELISA, Western blot, immunoprecipitation, and immunofluorescence assays.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Cells, Cultured
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Dogs
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Enzyme-Linked Immunosorbent Assay
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Female
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Fibroblasts / cytology
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Fibroblasts / metabolism
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HeLa Cells
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Humans
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Hybridomas
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Immunoblotting
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Immunoenzyme Techniques
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Immunoglobulin G / immunology*
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Immunoprecipitation
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Kidney / cytology
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Kidney / metabolism
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Mice
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Mice, Inbred BALB C
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NIH 3T3 Cells
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology*
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Recombinant Proteins / metabolism
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Viral Proteins / immunology*
Substances
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Antibodies, Monoclonal
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Immunoglobulin G
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PB1-F2 protein, Influenza A virus
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Recombinant Proteins
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Viral Proteins