Cryopreservation of mouse gametes and embryos

Methods Enzymol. 2010:476:85-105. doi: 10.1016/S0076-6879(10)76006-7.

Abstract

As the use of genetically engineered mouse models continues to expand, the need to cryopreserve strains of mice increases in parallel in order to preserve these unique research resources and provide a low-cost alternative to maintaining the large inventory of strains. This chapter discusses methods for the cryopreservation of mouse embryos, sperm, and oocytes, and briefly discusses other requirements for implementing a successful cryopreservation program.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cryopreservation / instrumentation
  • Cryopreservation / methods*
  • Culture Media
  • Embryo Transfer / methods
  • Embryo, Mammalian*
  • Female
  • Fertilization in Vitro / instrumentation
  • Fertilization in Vitro / methods
  • Male
  • Mice
  • Oocytes* / cytology
  • Quality Control
  • Semen Preservation / instrumentation
  • Semen Preservation / methods*
  • Spermatozoa* / cytology

Substances

  • Culture Media