To evaluate the level of alpha-Tocopherol in seminal fluid of patients with low fertility, forty subjects with low fertility (17 with asthenospermia and 32 with oligoasthenospermia) and 21 subjects with normal sperm parameters were recruited into this study for assessing their alpha-Tocopherol seminal fluid level. The mean level of alpha-Tocopherol in subjects with normal sperm profile was 0.62 micromol/l compared to those with pathological profile such as asthenospermia (0.29 micromol/l) and oligoasthenospermia (0.28 micromol/l). The determination of alpha-Tocopherol in human seminal fluid provides useful information concerning the exploration of low fertility in Cote d'Ivoire.