Abstract
Tyrosine hydroxylase is studied in terms of adsorption behaviour on gold surfaces and various passivating layers. Results reveal differences in layer formation, where mercaptoundecanoic acid-coated gold shows the best potential in terms of adsorbed mass. Nanoparticles with this coating are subsequently tested for enzymatic activity, which remains at attenuated levels.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adsorption
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Enzymes, Immobilized / chemistry*
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Enzymes, Immobilized / metabolism
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Gold / chemistry*
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Humans
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Hydrogen-Ion Concentration
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Metal Nanoparticles / chemistry*
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Particle Size
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Protein Isoforms
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Protein Stability
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Tyrosine 3-Monooxygenase / chemistry*
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Tyrosine 3-Monooxygenase / metabolism
Substances
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Enzymes, Immobilized
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Protein Isoforms
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Recombinant Proteins
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Gold
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Tyrosine 3-Monooxygenase