Adsorption and bioactivity of tyrosine hydroxylase on gold surfaces and nanoparticles

Protein Pept Lett. 2010 Nov;17(11):1376-82. doi: 10.2174/0929866511009011376.

Abstract

Tyrosine hydroxylase is studied in terms of adsorption behaviour on gold surfaces and various passivating layers. Results reveal differences in layer formation, where mercaptoundecanoic acid-coated gold shows the best potential in terms of adsorbed mass. Nanoparticles with this coating are subsequently tested for enzymatic activity, which remains at attenuated levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Enzymes, Immobilized / chemistry*
  • Enzymes, Immobilized / metabolism
  • Gold / chemistry*
  • Humans
  • Hydrogen-Ion Concentration
  • Metal Nanoparticles / chemistry*
  • Particle Size
  • Protein Isoforms
  • Protein Stability
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Tyrosine 3-Monooxygenase / chemistry*
  • Tyrosine 3-Monooxygenase / metabolism

Substances

  • Enzymes, Immobilized
  • Protein Isoforms
  • Recombinant Proteins
  • Gold
  • Tyrosine 3-Monooxygenase