To better understand and observe craniofacial growth, researchers carry out longitudinal cephalometric studies on animals. The authors constructed a cephalostat specially designed to study craniofacial growth in rats. They describe and validate a cephalometric technique using this cephalostat. They carried out cephalometric radiography and analysis of 62 Wistar rats. By using this method, the authors were able to take repeated, reproducible intracranial, intramandibular and craniomandibular measurements of the rats. The proposed method is a useful tool for studying the craniofacial growth process.