[Effects of cocaine on activities of ATPase, LDH and SDH in mouse splenocytes]

Fa Yi Xue Za Zhi. 2010 Apr;26(2):81-3.
[Article in Chinese]

Abstract

Objective: To examine the effects of cocaine on the activities of ATPase, LDH and SDH in cultured mouse splenocytes in vitro.

Methods: The ATPase, LDH and SDH activities in mouse splenocytes were detected at day 7 after continuous culturing the mouse cells exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 microg/mL in vitro.

Results: The activities of ATPase, LDH and SDH in mouse splenocytes exposed to cocaine hydrochloride in final concentration of 10, 20 and 100 microg/mL were significantly decreased after continuous culturing for 7 days.

Conclusion: The present study demonstrated that cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes in vitro.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Cells, Cultured
  • Cocaine / administration & dosage
  • Cocaine / pharmacology*
  • Dose-Response Relationship, Drug
  • L-Lactate Dehydrogenase / metabolism*
  • Male
  • Mice
  • Mice, Inbred Strains
  • Spleen / cytology*
  • Spleen / drug effects
  • Spleen / enzymology*
  • Succinate Dehydrogenase / metabolism*

Substances

  • L-Lactate Dehydrogenase
  • Succinate Dehydrogenase
  • Adenosine Triphosphatases
  • Cocaine