The gene expression of the myocardial lipid droplet protein is highly regulated by PPARgamma in adipocytes differentiated from MEFs or SVCs

Biochem Biophys Res Commun. 2010 Aug 20;399(2):209-14. doi: 10.1016/j.bbrc.2010.07.056. Epub 2010 Jul 17.

Abstract

We demonstrate that expression of the myocardial lipid droplet protein (MLDP) and ERalpha observed in adipose tissues is undetectable in 3T3-L1 cells but detectable in mouse embryonic fibroblasts (MEFs) and stromal-vascular cells (SVCs) during adipocyte differentiation. MLDP gene expression in MEFs or SVCs is induced by treatment with a PPARgamma agonist or forced expression of PPARgamma, indicating that PPARgamma enhances MLDP expression during adipogenesis. PCR analyses reveal the dual expression of SREBP-1a and SREBP-1c in MEFs and SVCs as well as white adipose tissues unlike the predominant expression of SREBP-1a in 3T3-L1 cells. These results suggest that MEFs and SVCs are useful model cells for examining function of MLDP in lipid droplet formation and adipocyte differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology*
  • Adipocytes / metabolism
  • Adipogenesis / genetics*
  • Animals
  • Endothelium, Vascular / metabolism*
  • Estrogen Receptor alpha / genetics
  • Fibroblasts / metabolism*
  • Gene Expression Regulation*
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Mice
  • PPAR gamma / metabolism*
  • Sterol Regulatory Element Binding Protein 1 / genetics
  • Stromal Cells / metabolism

Substances

  • Estrogen Receptor alpha
  • Intracellular Signaling Peptides and Proteins
  • OXPAT protein, mouse
  • PPAR gamma
  • Srebf1 protein, mouse
  • Sterol Regulatory Element Binding Protein 1