Synthesis, DNA-binding and photocleavage of "light switch" complexes [Ru(bpy)2(pyip)]2+ and [Ru(phen)2(pyip)]2+

Spectrochim Acta A Mol Biomol Spectrosc. 2010 Oct 1;77(2):522-7. doi: 10.1016/j.saa.2010.06.030. Epub 2010 Jul 1.

Abstract

Two novel Ru(II) complexes [Ru(bpy)(2)(pyip)](2+)1 and [Ru(phen)(2)(pyip)](2+)2 (bpy=2,2'-bipyridine; phen=1,10-phenanthroline; pyip=2-(pyridine-2-yl)imidazo-[4,5-f][1,10]-phenanthroline), have been synthesized and characterized by elemental analysis, ES-MS, (1)H NMR, UV-Vis. The DNA-binding behaviors of both complexes were studied by spectroscopic methods and viscosity measurements. The results indicate that the two complexes can bind to CT-DNA in an intercalative mode, and also show that these two Ru(II) complexes can promote the photocleavage of pBR322 DNA. In addition, In the presence of Co(2+), the emission of DNA-[Ru(L)(2)pyip](2+) can be quenched, which exhibited the DNA "light switch" properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • DNA / metabolism
  • Intercalating Agents / chemistry
  • Light*
  • Molecular Structure
  • Phenanthrolines / chemistry*
  • Phenanthrolines / metabolism
  • Ruthenium / chemistry*
  • Ruthenium / metabolism

Substances

  • Intercalating Agents
  • Phenanthrolines
  • Ruthenium
  • DNA
  • 1,10-phenanthroline