Epiphany/Resilon and GuttaFlow are newly developed methods for obturation of the root canal system. Epiphany/Resilon is a thermoplastic, synthetic polymer-based root canal filling material which enables the bonding to the dentin root canal wall during root canal obturation. GuttaFlow is a cold flowable filling system for the obturation of root canals, combining sealer and gutta-percha in one product. The purpose of this study was to assess the leakage of the Epiphany/Resilon or GuttaFlow root canal filling compared with lateral condensation of gutta-percha. For this study were used 45 human extracted teeth, chemo mechanically prepared, divided into three groups and obturated with gutta-percha/AH Plus, Epiphany/Resilon and GuttaFlow. For dye penetration all teeth were centrifuged for three minutes at 30 g in 2% methylene blue and dissolved in 65% nitric acid for 3 days. The extracted methylene blue was determined with Photometer. Root Canal fillings with Epiphany/Resilon showed less dye penetration than lateral condensation of gutta-percha and GuttaFlow. Epiphany/Resilon is ideally suited as a root canal filling material.