Five known flavonoids (1-5) and three sesquiterpene lactones (6-8) are reported for the first time from the endemic species Centaurea sulphurea Willd. (Asteraceae). Compound 7 is a new heliangolide (6R, 7R, 8S, 3'R)-8alpha-(3',4'-dihydroxy-methylene-2'-butanoyloxy)-15-oxo-helianga-1 (10), 4(5), 11(13)-trien-6,12-olide, named as sulphurein.