Abstract
A method for patterning narrow lines of biomolecules onto nitrocellulose membranes using laboratory syringe pumps is described. One syringe pump is used to drive the biomolecule solution through a needle, while another modified syringe pump acts as a one-dimensional translation stage, moving the needle across the membrane much like a pen. This method consumes very small volumes of reagents, and is a viable option for laboratory-scale fabrication and prototyping of point-of-care rapid diagnostic test strips.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Immobilized / chemistry
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Antibodies, Immobilized / immunology
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Biosensing Techniques / economics
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Biosensing Techniques / instrumentation*
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Clinical Laboratory Techniques
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Equipment Design
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Gold / chemistry
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Humans
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Immunoassay / economics
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Immunoassay / instrumentation*
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Immunoglobulin G / chemistry
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Immunoglobulin G / immunology
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Metal Nanoparticles / chemistry
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Paper*
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Proteins / chemistry*
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Proteins / immunology
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Reagent Strips*
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Streptavidin / chemistry
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Streptavidin / immunology
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Syringes
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Time Factors
Substances
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Antibodies, Immobilized
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Immunoglobulin G
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Proteins
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Reagent Strips
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Gold
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Streptavidin