Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis

J Clin Microbiol. 2010 Sep;48(9):3392-4. doi: 10.1128/JCM.01109-10. Epub 2010 Jun 30.

Abstract

We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics*
  • Humans
  • Molecular Sequence Data
  • Mucorales / classification*
  • Mucorales / genetics
  • Mucorales / isolation & purification*
  • Mucormycosis / diagnosis*
  • Mycology / methods*
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA
  • Transition Temperature

Substances

  • DNA, Fungal

Associated data

  • GENBANK/HM234125
  • GENBANK/HM234126
  • GENBANK/HM234127
  • GENBANK/HM234128
  • GENBANK/HM234129
  • GENBANK/HM234130