Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry

Mass Spectrom Rev. 2010 Jul-Aug;29(4):606-50. doi: 10.1002/mas.20258.

Abstract

Mass spectrometric studies are now playing a leading role in the elucidation of lipopolysaccharide (LPS) structures through the characterization of antigenic polysaccharides, core oligosaccharides and lipid A components including LPS genetic modifications. The conventional MS and MS/MS analyses together with CID fragmentation provide additional structural information complementary to the previous analytical experiments, and thus contribute to an integrated strategy for the simultaneous characterization and correct sequencing of the carbohydrate moiety.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Aeromonas / chemistry
  • Bordetella / chemistry
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chemical Fractionation
  • Escherichia coli / chemistry
  • Escherichia coli K12 / chemistry
  • Klebsiella pneumoniae / chemistry
  • Lipid A / chemistry
  • Lipopolysaccharides / chemistry*
  • Mass Spectrometry*
  • Molecular Sequence Data
  • Molecular Structure
  • Moraxella / chemistry
  • Oligosaccharides / chemistry
  • Salmonella / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tandem Mass Spectrometry*
  • Vibrio / chemistry

Substances

  • Lipid A
  • Lipopolysaccharides
  • Oligosaccharides