An engineered riboswitch as a potential gene-regulatory platform for reducing antibacterial drug resistance

Xuli Feng; Libing Liu; Xinrui Duan; Shu Wang

doi:10.1039/c0cc00980f

An engineered riboswitch as a potential gene-regulatory platform for reducing antibacterial drug resistance

Chem Commun (Camb). 2011 Jan 7;47(1):173-5. doi: 10.1039/c0cc00980f. Epub 2010 Jun 30.

Authors

Xuli Feng¹, Libing Liu, Xinrui Duan, Shu Wang

Affiliation

¹ Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, PR China.

PMID: 20589309
DOI: 10.1039/c0cc00980f

Abstract

Synthetic riboswitch containing aptamer is constructed in E. coli to regulate the expression of β-lactamase through small molecule-aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Aptamers, Nucleotide / chemistry
Aptamers, Nucleotide / pharmacology*
Catalytic Domain / drug effects
Escherichia coli / drug effects*
Escherichia coli / enzymology
Escherichia coli / genetics
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Enzymologic / genetics
Genetic Engineering* / methods
Molecular Structure
Riboswitch* / drug effects
Theophylline / chemistry
Theophylline / pharmacology
beta-Lactam Resistance / drug effects*
beta-Lactam Resistance / genetics
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Aptamers, Nucleotide
Riboswitch
Theophylline
beta-Lactamases