A simple scanless two-photon fluorescence microscope using selective plane illumination

Jonathan Palero; Susana I C O Santos; David Artigas; Pablo Loza-Alvarez

doi:10.1364/OE.18.008491

A simple scanless two-photon fluorescence microscope using selective plane illumination

Opt Express. 2010 Apr 12;18(8):8491-8. doi: 10.1364/OE.18.008491.

Authors

Jonathan Palero¹, Susana I C O Santos, David Artigas, Pablo Loza-Alvarez

Affiliation

¹ ICFO-Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860 Castelldefels (Barcelona), Spain.

PMID: 20588695
DOI: 10.1364/OE.18.008491

Abstract

We demonstrate a simple scanless two-photon (2p) excited fluorescence microscope based on selective plane illumination microscopy (SPIM). Optical sectioning capability is presented and depth-resolved imaging of cameleon protein in C. elegans pharyngeal muscle is implemented.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caenorhabditis elegans / metabolism
Coumarins / metabolism
Lighting / instrumentation*
Luminescent Proteins / metabolism
Microscopy, Fluorescence / instrumentation*
Muscles / metabolism
Pharynx / metabolism
Photons*

Substances

Coumarins
Luminescent Proteins
coumarin