Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen

Parima Boontanom; Suradej Siripattanapipong; Mathirut Mungthin; Peerapan Tan-ariya; Saovanee Leelayoova

Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen

Southeast Asian J Trop Med Public Health. 2010 Mar;41(2):280-4.

Authors

Parima Boontanom¹, Suradej Siripattanapipong, Mathirut Mungthin, Peerapan Tan-ariya, Saovanee Leelayoova

Affiliation

¹ Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.

PMID: 20578509

Abstract

A modified set of primers was developed to increase the sensitivity of nested PCR amplification of glutamate dehydrogenase (gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set of primers had a significantly higher sensitivity (82%) than that of a previously published PCR primer set (53%).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Primers
Feces / parasitology
Genotype
Giardia lamblia / genetics*
Glutamate Dehydrogenase / genetics*
Humans
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Thailand

Substances

DNA Primers
Glutamate Dehydrogenase