Abstract
Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA / chemistry*
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DNA Damage
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Deoxyribonuclease I / chemistry*
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Endodeoxyribonucleases / chemistry*
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Hydrogen-Ion Concentration
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Magnetic Resonance Spectroscopy
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Manganese / chemistry*
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Metalloporphyrins / chemistry*
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Organophosphorus Compounds / chemistry*
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Phosphorus Radioisotopes
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Potassium
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Potassium Compounds*
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Sulfates
Substances
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Metalloporphyrins
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Organophosphorus Compounds
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Phosphorus Radioisotopes
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Potassium Compounds
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Sulfates
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Manganese
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potassium persulfate
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tetrakis(N-methyl-4-pyridiniumyl)porphine manganese(III) complex
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DNA
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Endodeoxyribonucleases
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Deoxyribonuclease I
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deoxyribonuclease II
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Potassium