31P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/KHSO5' in comparison with DNases I and II

Nucleic Acids Res. 1991 Jun 11;19(11):2835-9. doi: 10.1093/nar/19.11.2835.

Abstract

Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry*
  • DNA Damage
  • Deoxyribonuclease I / chemistry*
  • Endodeoxyribonucleases / chemistry*
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy
  • Manganese / chemistry*
  • Metalloporphyrins / chemistry*
  • Organophosphorus Compounds / chemistry*
  • Phosphorus Radioisotopes
  • Potassium
  • Potassium Compounds*
  • Sulfates

Substances

  • Metalloporphyrins
  • Organophosphorus Compounds
  • Phosphorus Radioisotopes
  • Potassium Compounds
  • Sulfates
  • Manganese
  • potassium persulfate
  • tetrakis(N-methyl-4-pyridiniumyl)porphine manganese(III) complex
  • DNA
  • Endodeoxyribonucleases
  • Deoxyribonuclease I
  • deoxyribonuclease II
  • Potassium