Inhibition of twisting of a green fluorescent protein-like chromophore by metal complexation

Chem Commun (Camb). 2010 Aug 21;46(31):5686-8. doi: 10.1039/b927313a. Epub 2010 Jun 21.

Abstract

Substitution of a pyridyl for the hydroxyphenyl moiety in the Green Fluorescent Protein analog p-hydroxybenzylidene-dimethylimidiazolinone produces a chromophore which "turns on" fluorescence in the presence of Zn(2+) or Cd(2+) ions. Such a phenomenon provides "proof of principle" for using GFP chromophores in a variety of sensing applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Benzyl Compounds / chemistry*
  • Cadmium / chemistry
  • Coordination Complexes / chemical synthesis
  • Coordination Complexes / chemistry*
  • Green Fluorescent Proteins / chemistry
  • Imidazolines / chemistry*
  • Ligands
  • Molecular Conformation
  • Spectrometry, Fluorescence
  • Zinc / chemistry

Substances

  • Benzyl Compounds
  • Coordination Complexes
  • Imidazolines
  • Ligands
  • p-hydroxybenzylidenedimethylimidazolinone
  • Cadmium
  • Green Fluorescent Proteins
  • Zinc