Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes

Kohsuke Honda; Shohei Maya; Takeshi Omasa; Ryuichi Hirota; Akio Kuroda; Hisao Ohtake

doi:10.1016/j.jbiotec.2010.06.008

Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes

J Biotechnol. 2010 Aug 2;148(4):204-7. doi: 10.1016/j.jbiotec.2010.06.008. Epub 2010 Jun 11.

Authors

Kohsuke Honda¹, Shohei Maya, Takeshi Omasa, Ryuichi Hirota, Akio Kuroda, Hisao Ohtake

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan. honda@bio.eng.osaka-u.ac.jp

PMID: 20547190
DOI: 10.1016/j.jbiotec.2010.06.008

Abstract

Six thermophilic enzymes from Thermus thermophilus were used to construct an 'artificial bio-synthetic pathway' for the production of 2-deoxyribose 5-phosphate from fructose. By a simple operation using six recombinant Escherichia coli strains producing the thermophilic enzymes, respectively, fructose was converted to 2-deoxyribose 5-phosphate with a molar yield of 55%.

MeSH terms

Biomimetics / methods*
Escherichia coli / physiology*
Feasibility Studies
Fructose / chemistry*
Genetic Engineering / methods*
Lyases / chemistry*
Lyases / genetics
Lyases / metabolism
Recombinant Proteins / metabolism
Ribosemonophosphates / chemical synthesis*
Signal Transduction / physiology
Thermus thermophilus / enzymology*
Thermus thermophilus / genetics

Substances

Recombinant Proteins
Ribosemonophosphates
Fructose
2-deoxyribose 5-phosphate
Lyases